Highly selective amperometric biosensor for uric acid determination in real samples
نویسندگان
چکیده
منابع مشابه
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H₂O₂ at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 - 8.5×10-5 M and ...
متن کاملAmperometric biosensor for ascorbic acid
A L-ascorbic acid biosensor based on ascorbate oxidase has been developed. The enzyme was extracted from the mesocarp of cucumber (Cucumis sativus) by using 0.05 mol L phosphate buffer, pH 5.8 containing 0.5 mol L NaCl. After the dialysis versus phosphate buffer 0.05 mol L pH 5.8, the enzyme was immobilized onto nylon net through glutaraldehyde covalent bond. The membrane was coupled to an O 2 ...
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The first enzyme-based biosensors capable of determining glycolic acid in various complex matrixes, such as cosmetics, instant coffee, and urine, are presented in this paper. Two separate designs, both based on a three-membrane configuration consisting of an inner cellulose acetate membrane (CA) and an outer polycarbonate membrane (PC), which sandwich a membrane bearing the biomolecule(s), are ...
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ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
15 صفحه اولAscorbic Acid Determination by an Amperometric Ascorbate Oxidase-based Biosensor
The aim of this work is to develop a method for ascorbic acid determination in some fruit juices, by using an ascorbate oxidase-based amperometric enzyme electrode. The enzyme was immobilized on a nylon (Biodyne A) membrane by glutaraldehyde linking. The enzyme-membrane was consecutively attached to the polyethylene membrane of a Clark oxygen electrode, which functioned as transducer. The analy...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 2017
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.00094a